Application

Peptide Inhibition Analysis: A 1:500 dilution from a representative lot was used with NIH3T3 Nuclear Extract for peptide block analysis.

Anti-phospho BAF60C (Ser247), Cat. No. ABE1298, is a highly specific rabbit polyclonal antibody that targets SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 3 (BAF60C) phosphorylatd on Serine 247 and has been tested for use in Peptide Inhibition & WB.

General description

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 3 (UniProt: Q6STE5; also known as 60 kDa BRG-1/Brm-associated factor subunit C, BRG1-associated factor 60C, BAF60C) is encoded by the SMARCD3 (also known as BAF60C) gene (Gene ID: 6604) in human. BAF60C plays a key role in ATP dependent nucleosome remodeling by SMARCA4 containing complexes. Two isoforms of BAF60C have been reported that are produced by alternative splicing. Both isoforms are expressed in brain, heart, kidney, placenta, prostate, salivary glands, spleen, testis, thyroid, trachea, and uterus. However, isoform 1 is expressed also in skeletal muscle and adipose tissue. BAF60C belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. BAF60C is also a component of nuclear Notch signaling and is required for the establishment of left-right asymmetry. Loss of BAF60C can lead to defects in the establishment of the left-right asymmetry cascade in mouse and zebrafish. It also serves a specific chromatin remodeling component for lipogenic gene transcription in liver. In response to insulin, it is phosphorylated at serine 247 by atypical PKC zeta//lambda, which causes translocation of BAF60C to the nucleus and allows a direct interaction of BAF60C with upstream stimulatory factor-1 (USF-1). (Ref.: Takeuchi, JK., et al. (2007). Proc. Natl. Acad. Sci. USA 104(3); 846-851; Wang, Y., et al. (2012). Mol. Cell 49(2); 283-297).

Immunogen

KLH-conjugated linear peptide corresponding to 9 amino acids surrounding phosphorylated serine 247 of human BAF60C. The immunogen is conserved between both isoforms.

Other Notes

Concentration: Please refer to lot specific datasheet.

Quality

Evaluated by Western Blotting in NIH3T3 Nuclear Extract.

Western Blotting Analysis: A 1:500 dilution of this antibody detected phospho BAF60C (Ser247) in NIH3T3 Nuclear Extract.

Specificity

This rabbit polyclonal antibody detects murine BAF60C phosphorylated on Serine 247.

Target description

~55 kDa observed. Uncharacterized bands may be observed in some lysate(s).